The 32X version of Doom was published by Sega in 1994. The game features most of the maps from first two episodes and lacks the famed BFG9000 (which only appears in the third episode that is not included in the 32X version), although it is documented in games manual. However, a cheat code can give the weapon to the player. This version also has no multiplayer mode. The game disappointed many fans since it did not play in a full screen, was missing many levels from other versions of the game, and only had the front sprite for the monsters.
Due to poor use of the YM2612 FM synthesis chip in the Sega Genesis, this version's soundtrack is noticeably inferior to that of other versions. Many sound effects are also missing. As with the Jaguar version, the levels have been edited and the game does not feature the Cyberdemon, Spiderdemon or the Spectre (unlike the BFG9000, these foes are also correctly, not mentioned in the manual). There is also no way to save games (although there is a level select option that allows the player to start on any of the first fifteen levels. However, if the level select option is used, players do not see the real ending to the game).
Like in the Jaguar version, circlestrafing is not possible in this version due to the use of a "strafe on" key rather than separate left and right strafe keys.
- If the player uses a cheat code, a DOS prompt (C:>) appears after the credits following level 15, instead of the ending. However, this happens too if any levels are skipped. The game's manual states the game will wrap around back to level 1 if any are skipped, but this doesn't happen.
- Some status bar face graphics are flipped left to right with respect to the originals, so that when the Doomguy takes damage, the part in his hair appears to jump from side to side.